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Q. M. Wang, S. H. Sun, Z. L. Hu, F. J. Zhou, M. Yin, C. J. Xiao and J. C. Zhang, “Epitope DNA Vaccines against Tuberculosis: Spacers and Ubiquitin Modulates Cellular Immune Responses Elicited by Epitope DNA Vaccine,” Scandinavian Journal of Immunology, Vol. 60, No. 3, 2004, pp. 219-225. doi:10.1111/j.0300-9475.2004.01442.x

has been cited by the following article:

• TITLE: Ub Combination Enhanced Cellular Immune Response Elicited by HSP65 DNA Vaccine against Mycobacterium tuberculosis

  AUTHORS: Qingmin Wang, Chengxiang Lei, Qiuhong Liu

  KEYWORDS: Mycobacterium tuberculosis; HSP65; Ubiquitin; Immune Response

  JOURNAL NAME: World Journal of Vaccines, Vol.3 No.3, August 20, 2013

  ABSTRACT: This study observed the immune response induced by a HSP65 DNA vaccine fused with UbGR against Mycobacterium tuberculosis. BALB/c mice were inoculated with HSP65 DNA vaccine, UbGR-fused HSP65 DNA vaccine (Ub-GR-HSP65) and blank vector respectively. HSP65 DNA vaccine elicited a Thl-polarized immune response. The Thl-type cytokine (IFN-γ) and proliferative T cell responses from spleen were improved significantly in UbGR-HSP65 group, compared with those in HSP65 DNA vaccine group. Furthermore, this fusion DNA vaccine also led to an increased ratio of IgG2ato IgGl and the cytotoxicity of T cells. IFN-γ intracellular staining of splenocytes indicated that UbGR-HSP65 fusion DNA vaccine could activate CD4+ and CD8+ T cells, with much higher CD8+ T cells. Thus, this study demonstrated that the UbGR fusion could improve HSP65-specific cellular immune responses, which is helpful to protect against TB infection.