TITLE:
Evaluation of Transgenic Nicotiana tabacum with dehE Gene Using Transposon Based IRAP Markers
AUTHORS:
Yilmaz Kaya, Sibel Yilmaz, Nermin Gozukirmizi, Fahrul Huyop
KEYWORDS:
Nicotiana tabacum; Dehalogenase E; Tto1 Retrotransposon; IRAP; Transgenics
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.8A,
August
16,
2013
ABSTRACT: In the present study, five
genetically modified herbicide tolerant Nicotiana tabacum cv. TAPM24
plants with a constructed vector pCAMBIA1301a carrying dehalogenase E (dehE)
gene were compared with three non-transgenic controls using Tto1 retrotransposon specific IRAP
markers. dehE gene was originally characterized in Rhizobium sp.
and it produced an enzyme which degraded the Dalapon herbicide. IRAP protocol
was applied on transgenic and non-transgenic plants to investigate the
retrotransposon based genetic variation which may appear during transformation.
Polymorphism rates were calculated as 0%-20% from IRAP-PCR products
among all plant samples. These results show that transformation of tobacco
plant with the dehE gene may cause Tto1 retrotransposon alterations
appearing as different band profiles. The findings are expected to contribute
to genetic engineering studies to obtain better results and also to understand
how transposons contribute to features such as transgenesis. In our knowledge,
this is one of the first experimental data of transgenic N.
tabacum engineered with dehE gene originated Rhizobium sp. in
terms of retrotranposon based variation.