TITLE:
Influence of maternal experimental hypothyroidism on quantitative-qualitative indicator of rat progeny skin mast cells in age aspect according to histochemical investigation results and on the base of lectins GNA and PNA receptors cytotopography
AUTHORS:
Khrystyna Strus, Antonina Yashchenko, Olena Smolkova, Olga Nakonechna
KEYWORDS:
Skin; Rat; Hypothyroidism; Lectin Histochemistry; Mast Cells
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.8,
August
16,
2013
ABSTRACT: Amount of thyroid pathology patients in Ukraine increased
3.7 times from 0.9 to 3.5 per 1000 population within a decade. The main reason
of most of organs damage associated with hypothyroidism is decreased synthesis
of number of cellular enzymes because of thyroid hormones deficiency. Mast
cells (MC) play leading role in inflammatory processes, allergic reactions
and in autoimmune diseases pathogenesis, since they produce various cytokines.
Influence of maternal hypothyroidism on the progeny skin histogenesis and MC
correlation is poorly studied. Hypothyroid condition was modeled in Wistar
female rats by adding thyreostatic drug
mercazolilum (methimazole) 5 mg/kg body mass. Thyroid glands and progeny
skin pieces from the back on the 1, 10, 20 and 40 postnatal development days
were fixed in 4% neutral formalin and embedded in paraffin. For MC detection
slides were stained by Bismark brown, alcian blue (pH 2.5), toluidine blue.
D-Man and β-DGal carbohydrate determinants
were studied by use of GNA and PNA lectins labeled with horseradish peroxidase.
Lectin receptors visualization was conducted in3’3-diaminobenzidine tetrahydrochloride system in Н2О2 presence. Counting the MC number and thyroid glands’ morphometric parameters
were conducted on 5 μm thin sections by using UTHSCSA “Image Tool for Windows
Version2.00”(USA)
computer program. Statistical analysis was performed using Student t-test. Body
mass increase, changes in thyroid cells parameters and colloid structure were
stated in experimental animals. The biggest MC amount was detected in
control animals skin on the 1 day of postnatal development, slight decrease
on 10 day and gradual increase till 40 day. MC amount with signs of degranulation
increased in experimental animals skin at all stages of the research.
Simultaneously, D-Man and β-DGal glycopolymers expression similarity was
noted on MC surface. According to MC quantitative-qualitative indicators in
skin, hypothyroid female rat progeny should be included into the risk group
of immune status change and allergic reactions beginning.