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J. D. Newman, “Natural Products as Leads to Potential Drugs: An Old Process or the New Hope for Drug Discovery?” Journal of Medicinal Chemistry, Vol. 51, No. 9, 2001, pp. 2589-2599. doi:10.1021/jm0704090

has been cited by the following article:

• TITLE: Hyphal Morphology and Elongation Alterations in Aspergillus nidulans Provoked by the Diterpene Kaurenoic Acid

  AUTHORS: Janice A. Rafael, Nilton S. Arakawa, Sérgio R. Ambrosio, Fernando B. Da Costa, Suraia Said

  KEYWORDS: Aspergillus nidulans; Calcium Gradient; Fungistatic Activity; Kaurenoic Acid

  JOURNAL NAME: Advances in Microbiology, Vol.3 No.5, August 16, 2013

  ABSTRACT: Kaurenoic acid (KA), a kaurane-type diterpene extracted from leaves of Mikania hirsutissima, was previously reported as an inhibitor of vascular contractility mainly by blocking extracellular Ca2+ influx. The compound is known for several other biological activities such as antiparasitic, antispasmodic and antibacterial activity. The aim of the present study was to investigate the effect of KA on Aspergillus nidulans. KA (0.3 mM) showed fungistatic activity against A. nidulans with visible hyphal elongation and morphology damage. These effects were reverted by CaCl2 addition showing that KA interferes with intracellular Ca2+ gradient in A. nidulans. This is the first report on the mechanism of action of KA involving calcium levels by altering the elongation of fungi hyphae.