TITLE:
Comparison of Positions of QTLs Conferring Resistance to Xanthomonas campestris pv. campestris in Brassica oleracea
AUTHORS:
Nazmoon Naher Tonu, Md. Asad-ud Doullah, Motoki Shimizu, Md. Masud Karim, Takahiro Kawanabe, Ryo Fujimoto, Keiichi Okazaki
KEYWORDS:
Black Rot; Disease Resistance; QTL; Xanthomonas campestris pv. campestris
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.8A,
August
15,
2013
ABSTRACT: Black
rot, caused by Xanthomonas campestris pv. campestris (Xcc)
is possibly the most important disease of Brassica worldwide. To compare
chromosomal positions of Xcc resistance loci in Brassica oleracea between
the present and published studies and to develop marker assisted selection
(MAS) to resistance against Xcc race 1, we constructed a B. oleracea map,
including pW, pX and BoCL markers that were closely linked to previously
reported Xcc resistance QTLs. We also analyzed Xcc resistance
QTLs by improving our previously reported map derived from the cross of a
susceptible double-haploid line (GC P09) with a resistant double-haploid line
(Reiho P01). In the nine linkage groups obtained (C1-C9), the major QTL, XccBo(Reiho)2,
was derived from Reiho with a maximum LOD score (7.7) in C8. The QTL (LOD 4.4)
located in C9, XccBo(GC)1 was derived from the susceptible GC.
The other QTL (LOD 4.4), XccBo(Reiho)1, was found in C5. Based on
common markers, it was possible to compare our finding Xcc resistance
QTLs with the B. oleraceaXcc loci reported by previous authors; XccBo(Reiho)1
and XccBo(GC)1 may be identical to the Xcc resistance QTLs
reported previously or a different member contained in the same resistance gene
cluster. Our map includes public SSR markers linked to Xcc resistance
genes that will promote pyramiding Xcc resistance genes in B.
oleracea. The present study will also contribute to a better understanding
of genetic control of Xcc resistance.