TITLE:
Effect of mango seed kernel extract on the adipogenesis in 3T3-L1 adipocytes and in rats fed a high fat diet
AUTHORS:
Mai Kobayashi, Isao Matsui-Yuasa, Maiko Fukuda-Shimizu, Yoshinobu Mandai, Masaki Tabuchi, Hiroshi Munakata, Akiko Kojima-Yuasa
KEYWORDS:
Mago Seed Kernel; Glycerol 2-Phosphate Dehydrogenase (GPDH); Peroxisome Proliferator-Activated Receptor γ (PPARγ); High Fat Diet-Induced Obesity Rat Model; 3T3-L1 Adipocytes
JOURNAL NAME:
Health,
Vol.5 No.8C,
August
13,
2013
ABSTRACT: Mangoes (Mangifera indica L.) are one of the most
important tropical foods. The seed is one of the main by-products of mango
processing. Therefore, it is important to find an economically viable use for
this waste (e.g., as a food additive or supplement with high nutraceutical
value). We investigated the anti-obesity effects of mango seed kernel extract
with hot water (MSKE-W) in 3T3-L1 adipocytes and in a high fat diet
(HFD)-induced obesity rat model. MSKE-W caused a significant decrease in the
activity of glycerol 2-phosphate dehydrogenase in 3T3-L1 adipocytes without
eliciting cell cytotoxicity and inhibited cellular lipid accumulation through
down-regulation of transcription factors such as PPARγ and C/EBPα. In the
animal model, rats fed an HFD containing 1% MSKE-W gained less weight than rats
fed an HFD alone. The visceral fat mass in rats fed an HFD containing 1% MSKE-W
tended to be lower than that in rats fed an HFD alone. Furthermore,
histological examination of rat livers from an HFD showed steatohepatitis. However,
rats on an HFD containning 1% MSKE-W showed no histopathological changes in
liver tissue. Our results indicate that MSKE-W influences anti-obesity effects,
both in vitro and in vivo, and suggest that MSKE-W
provides a novel preventive potential against obesity.