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S. Sakuanrungsirikul, P. Sarawat, P. Wongtiem, S. Sarakarn, V. Khonsantear and R. Khantahad, “Molecular Characterization of the CIAT Cassava Core Collection and the Thai and Lao Germplasm Collection Using ISSR-Touchdown PCR,” Proceedings of the 8th Regional Workshop, Vientiane, 20-24 October 2008.

has been cited by the following article:

• TITLE: Identification of Genetically Distinct Cassava Clones from On-Farm Plantations to Widen the Thai Cassava Breeding Gene Pool

  AUTHORS: Preeya P. Wangsomnuk, Benjawan Ruttawat, Prapit Wongtiem

  KEYWORDS: Cassava; Distinctness; SSR; Ex Situ Conservation; Core Collection

  JOURNAL NAME: American Journal of Plant Sciences, Vol.4 No.8, July 30, 2013

  ABSTRACT: Cassava (Manihot esculenta Crantz) is one of the most important economic crops in Thailand. However, the Thai cassava breeding gene pool was genetically narrow with only 11 distinct landraces. An attempt was made here to characterize 266 cassava clones collected from 80 farms in eight provinces using 35 SSR markers. A total of 365 polymorphic alleles were detected in the assayed samples. The molecular analysis of variance revealed that a large SSR variance (19.8%) was present among the farm samples. The genetic relationships of the 266 farm samples revealed by the principal coordinate analysis confirmed the large SSR variation observed among the collected cassava samples. The average dissimilarity (AD) of a cassava sample against the other 265 samples was calculated and the AD values obtained ranged from 0.256 to 0.502 with a mean of 0.319. Based on these AD values, a set of 50 unique cassava samples with AD values of 0.346 or higher was assembled from the on-farm samples to widen the genetic base of the Thai cassava breeding gene pool.