Article citationsMore>>

J. Watanabe, T. Oki, J. Takebayashi, K. Yamasaki, Y. Takano-Ishikawa, A. Hino and A. Yasui, “Method Validation by Interlaboratory Studies of Improved Hydrophilic Oxygen Radical Absorbance Capacity Methods for the Determination of Antioxidant Capacities of Antioxidant Solutions and Food Extracts,” Analytical Sciences, Vol. 28, No. 2, 2012, pp. 159-165. doi:10.2116/analsci.28.159

has been cited by the following article:

• TITLE: Evaluation for Antioxidative Properties of Phlorotannins Isolated from the Brown Alga Eisenia bicyclis, by the H-ORAC Method

  AUTHORS: Yohei Fujii, Reiji Tanaka, Hideo Miyake, Yutaka Tamaru, Mitsuyoshi Ueda, Toshiyuki Shibata

  KEYWORDS: Antioxidants; Brown Alga; Dieckol; Eisenia bicyclis; Fucofuroeckol A; H-ORAC Assay; Phlorotannins

  JOURNAL NAME: Food and Nutrition Sciences, Vol.4 No.8A, July 29, 2013

  ABSTRACT: The antioxidative properties of phlorotannins isolated from the brown alga Eisenia bicyclis were measured using the H-ORAC (Hydrophilic Oxygen Radical Absorbance Capacity) method. The ORAC values of phloroglucinol and it’s oligomers: eckol, fucofuroeckol A, phlorofucofuroeckol A, dieckol, 8,8’-bieckol, were 2.57 ± 0.14, 4.97 ± 0.36, 9.82 ± 0.70, 8.97 ± 0.89, 10.22 ± 0.85, 8.62 ± 0.92 μmol Trolox equivalent/μmol, respectively. With the exception of eckol, the ORAC values of tested phlorotannins were higher than those of the well-known antioxidants (epigallocatechin gallate, resveratrol and L-ascorbic acid) used as positive controls. As a result of comparing with known ORAC values, it was found that the dieckol and fucofuroeckol A had stronger antioxidant activity than representative polyphenols (e.g., kaempferol, quercetin, myricetin and chlorogenic acid) derived from terrestrial plants.