TITLE:
The profiles of lipid peroxidation and anti-oxidant activities in gestational diabetes mellitus & normal pregnancies in Nigerian population
AUTHORS:
Adetunji O. Adeniji, Dolapo P. Oparinde
KEYWORDS:
Lipid Peroxidation; Anti-Oxidant Status; Gestational Diabetes Mellitus; Malondialdehyde
JOURNAL NAME:
Open Journal of Obstetrics and Gynecology,
Vol.3 No.6,
July
26,
2013
ABSTRACT: Purpose:
To compare the Lipid peroxidation and Total antioxidant status in women with
gestational diabetes mellitus and normal pregnancy in our environment. Materials
& Methods: This was a 2-year, cross sectional, case control study of 25 gestational
diabetes mellitus (GDM) and 75 matched normal pregnant women at Ladoke Akintola
University of Technology (LAUTECH) Teaching Hospital, Osogbo, Nigeria. The
study protocol was reviewed and approved by our Institutional Ethical Review Committee
and all participants gave their consents. The fasting serum of recruited
patients were analysed for lipid peroxidetion product malondialdehyde(MDA), based
on MDA reaction with thiobarbituric acid (TBA), with rapid, sensitive and
specific Thiobarbituric Acid (TBA) assay, while the Total Anti-oxidant status
(TAS) was determined using the capacity of the biological fluids to inhibit the
production of thiobarbituric acid reactive substances (TBARS) from sodium
benzoate under the influence of the free oxygen radicals derived from
Fenton’s reaction. Results: The mean serum MDA was significantly higher in the
GDM group (3.64 nmol/mL) than the value recorded (1.33 nmol/ mL) in the control
group (P vs. 0.66 nmol/mL) was noticed in the GDM
group (P