TITLE:
Cellulase Producing Bacteria from the Wood-Yards on Kallai River Bank
AUTHORS:
Sasidharan Sreedevi, Sreedharan Sajith, Sailas Benjamin
KEYWORDS:
Cellulase; Carboxy Methyl Cellulose; Dinitrosalicylic Acid; Optimization; Submerged Fermentation
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.4,
July
18,
2013
ABSTRACT: This study evaluates the influence of
growth parameters such as pH, temperature, Carboxy Methyl Cellulose (CMC)
concentration and agitation on cellulase
production from three bacterial strains, viz., Achromobacter xylosoxidans BSS4, Bacillus sp. BSS3 and Pseudomonas sp. BSS2 isolated from the
wood-yards on Kallai river bank in Kerala. Production of cellulase by these
isolates was detected using basal salt medium (BSM) with 0.5% CMC as supplement, and
CMCase activity was confirmed by iodine test. Dinitrosalicylic acid method was
employed for assaying the cellulase production by measuring the amount of
glucose liberated in μmol/mL/min. Maximum enzyme production from Pseudomonas sp. BSS2 was at pH 8, 37℃ with 1% CMC
and 150 rpm, and cellulase production increased from initial 49.84 U/mL to
91.28 U/mL after optimization. The highest enzyme activity from Bacillus sp. BSS3 was at pH 9, 37℃ with 1% CMC,
150 rpm, and cellulase production increased from initial 26.05 U/mL to 104.68
U/mL after optimization. The maximum enzyme production from A. xylosoxidans BSS4 was at pH 7, 40℃ with 0.5%
CMC and 150 rpm, and cellulase production increased from initial 55.28 U/mL to
68.37 U/mL after optimization. Thus among the three isolates, Bacillus sp. BSS3 showed maximum enzyme
yield which can be explored for further scale up studies with an industrial
perspective.