TITLE:
In Vitro Mass Scale Propagation of Wild Cymbidium lowianum with a Rare and Endangered Plant
AUTHORS:
Yuying Wang, Zhilin Li, Liping Huang, Jun Su
KEYWORDS:
Wild Cymbidium lowianum; Seed Germination; Propagation; Protocorm; Multiple Shoots
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.7,
July
17,
2013
ABSTRACT: The wild Cymbidium lowianum, a national-level
rare and endangered species of Orchid, is an excellent garden plant with
ornamental flowers with striking, deep red lips in a V-shaped formation. The
objective of this study was to establish a micropropagation protocol system
via immature seeds of wild Cymbidium
lowianum, evaluate the Murashige and Skoog (MS), half-strength Murashige
and Skoog (1/2 MS) medium, 6-benzylaminopurine (BA),
a-napthaleneacetic acid (NAA), organic additions activated charcoal (AC) and
banana pulp (BP) effects on the different morphogenesis (seed germination, multiple
shoot and rooting) in vitro. The
optimal combination for the germination of seed was 1.0 mg·L-1 BA
with 0.5 mg·L-1 NAA in 1/2 MS, and addition 0.3%AC, which resulted
in 95% seed germination in 90 days. The best formulation for multiple shoot was 1/2 MS medium containing 2.5 mg·L-1 BA, 0.5 mg·L-1 NAA
and addition 8% BP in which produced 19.8 shoots per protocorm in 60 days. Multiple
shoots were cut and rooted in 1/2 MS supplemented with 1.5 mg·L-1 NAA,0.1
mg·L-1 BA and
0.3% AC, roots initiated 20 days after culture, the rooting percentages reached
to 100%, in which 4.7 per shoot produced roots in 60 days. The survival rate of
plantlet was up to 92% in moss after 30 days. This finding reveals that it is
possible to obtain in vitro culture of Cymbidium
lowianum using immature seeds in asymbiotic culture.