TITLE:
Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real-time polymerase chain reaction
AUTHORS:
Sepideh Seghatoleslami, Lars Ohlsson, Kristina Hamberg, Peter Carlsson, Dan Ericson, Lennart Ljunggren
KEYWORDS:
Streptococcus mutans; FTATM Elute Cards; Real-Time PCR
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.3 No.3,
July
17,
2013
ABSTRACT: Dental caries is a localized, transmissible, pathological infection process that ends up in the destruction of hard dental tissue.
Numerous reports have shown the close relationship between salivary levels of Streptococcus mutans and dental
caries. As S. mutans, is considered
to be the principle etiological agent of dental caries, the development of a
quick and convenient method for detection and quantification of these bacteria
from patient saliva samples would simplify diagnosis and treatment. The
purpose of this study was to compare a new means of quantifying bacteria using
FTATM Elute cards and Real-Time Polymerase Chain Reaction to a
conventional culture-based assay using oral S.
mutans as a model sample. A total of 60 different saliva samples were
investigated. The results show a significant negative correlation between the
two methods, with a correlation coefficient of -0.577
(Spearman’s Correlation) and p