TITLE:
Polydopamine Particles Effect on Melanoma Cells Proliferation and Melanin Secretion
AUTHORS:
Sandy Eap, Alice Ferrand, Valérie Machi, Vincent Ball, Olivier Huck, Nadia Benkirane-Jessel
KEYWORDS:
Polydopamine Particles; Polyelectrolyte Multilayers Films; Melanin; Melanoma Cells
JOURNAL NAME:
Advances in Chemical Engineering and Science,
Vol.3 No.3A1,
July
12,
2013
ABSTRACT: Melanin is a biopolymer implicated in the
protection of cellular membranes and DNA produced by melanocytes. This pigment
has a dual role and should be considered as a photo-protector and as a
photosensitizer due to its interaction with UV. The design of multifunctional
and biologically responsive coatings is of major interest in modern
biomaterials science. The aim of this study is not only to characterize the
deposition of multilayered polyelectrolytes films made from polydopamine
particles and polyamines like poly-(L-lysine hydrobromide) (PLL), but also to
evaluate melanoma cells activity in terms of proliferation and their capacity
to stimulate melanin secretion. One could expect that the presence of a melanin
like material in the film may have a positive or a negative feedback on the
melanin biosynthesis and consequently on melanoma development. Some comparisons are also done with pure polydopamine grains in suspension in the cell
culture medium, to investigate if the immobilization of the polydopamine grains
has an influence on their bioactivity.