TITLE:
Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of Leishmania Genus Parasites
AUTHORS:
Pedro Rodríguez, Humberto Rojas, Miguel Medina, Jazzmin Arrivillaga, Yosue Francisco, Fracehuli Dager, Vincent Piscitelli, Manuel Caetano, Alberto Fernández, Jimmy Castillo
KEYWORDS:
Leishmania; Nanoparticles; Surface Plasmon Resonance; gp63 Protein
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.7A,
July
12,
2013
ABSTRACT: In this work we present the preparation and functionalization
of gold nanoparticles (AuNPs) for the detection of presence of gp63 glycoprotein
in the surface of Leishmania genus parasites.
AuNPs were prepared by induced laser ablation in a clean and biologically suitable
media. The nanoparticles were functionalized with anti-gp63 lgG antibody in order
to study the interaction with the glycoprotein component gp63 (63 kDa) present on
the membrane surface of Leishmania genus parasites.
The functionalized AuNPs showed potential as a spectrometric indicator of the parasite
existence, both by the detection of the presence of the gp63 in solution and through the specific interaction with
the parasites in vitro. The specificity
of the study opens a new line of research on the use of modified nanoparticles in
the development of a fast and easy assay for Leishmaniosis diagnostics.