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Y. Iketaki, T. Watanabe, N. Bokor and M. Fujii, “Investigation of the Center Intensity of First-and Second-Order Laguerre-Gaussian Beams with Linear and Circular Polarization,” Optics Letters, Vol. 32, No. 16, 2007, pp. 2357-2359. doi:10.1364/OL.32.002357

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• TITLE: A Low Cost and Versatile STED Superresolution Fluorescent Microscope

  AUTHORS: Daniel McBride, Chin Su, Jun Kameoka, Stanislav Vitha

  KEYWORDS: Stimulated Emission-Depletion Microscopy; STED

  JOURNAL NAME: Modern Instrumentation, Vol.2 No.3, July 12, 2013

  ABSTRACT: A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipments used are given. The excitation and depletion beams are extracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.