Article citationsMore>>
M. Bates, B. Huang, G. Dempsey and X. Zhuang, “Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes,” Science, Vol. 317, No. 5845, 2007, pp. 1749-1753. doi:10.1126/science.1146598
has been cited by the following article:
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TITLE:
A Low Cost and Versatile STED Superresolution Fluorescent Microscope
AUTHORS:
Daniel McBride, Chin Su, Jun Kameoka, Stanislav Vitha
KEYWORDS:
Stimulated Emission-Depletion Microscopy; STED
JOURNAL NAME:
Modern Instrumentation,
Vol.2 No.3,
July
12,
2013
ABSTRACT: A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipments used are given. The excitation and depletion beams are extracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.