TITLE:
Characterization of Microsatellite Markers and Their Application to Genetic Diversity Analysis of Brachypodium sylvaticum var. breviglume from Yunnan, China
AUTHORS:
Xinchun Mo, Ju Gao, Lizhi Gao
KEYWORDS:
Brachypodium sylvaticum var. breviglume; Microsatellites; Characterization; Genetic Diversity
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.7,
July
11,
2013
ABSTRACT: Southwestern China is abundant in a large species diversity of grasses and Brachypodium sylvaticum var. breviglume is unquestionably appropriate
to serve as an ideal model to investigate the adaptation, evolution and
diversification of grasses in this region. In this study, a total of 37
microsatellite markers were screened and genetic variation was estimated for
100 individuals from the five natural populations of the species. Our results
showed that seven of them were polymorphic for the five studied populations, while
the other thirty were monomorphic. These seven SSR loci exhibited a high level
of genetic diversity among populations, i.e. allele number varied from 5 to 24, with an average of 13.29; expected heterozygosity
(He) ranged from 0.439 to 0.561, with
an average of 0.488; percentage of polymorphic loci (PPL) ranged from 85.71% to 100%, with an average of 97. 14%. The
FCA and UPGMA analyses revealed that the five populations were divided into
three clusters. Our results indicate that these newly characterized SSR markers
are useful for the exploration of genetic diversity and evolutionary history of
the B. sylvaticum var. breviglume populations in Yunnan, China.