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Johns, J., Gold, H., Leinbach, R., Yasuda, T., Gimple, L., Werner, W., Finkelstein, D., Newell, J., Ziskind, A. and Collen, D. (1988) Prevention of coronary artery reocclusion and reduction in late coronary artery stenosis after thrombolytic therapy in patients with acute myocardial infarction. A randomized study of maintenance infusion of recombinant human tissue-type plasminogen activator. Circulation, 78, 546-556.

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• TITLE: Enzyme electrophoresis method in analysis of active components of haemostasis system

  AUTHORS: Ludmila Ostapchenko, Oleksiy Savchuk, Nataliia Burlova-Vasilieva

  KEYWORDS: Substrate-Containing Electrophoresis; Enzyme Electrophoresis; Haemostasis; Proteins Activity; Proteins Identification

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.2 No.1, February 24, 2011

  ABSTRACT: The novel modifications of substrate-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis that can be used for the detection of proteases and its activators are reported. The protease/activator samples were separated on a protein substrate-SDS-polyacrylamide gel. To detect plasminogen activators fibrinogen and Glu-plasminogen were incorporated into the SDS-PAG followed by 1 h incubation at 37?C in thrombin solution (1 NIH/ml). After electrophoresis the gel was stained according to the standard protocol. To detect fibrin-unspecific plasminogen activators from snake venom incubation in thrombin solution was substituted for 12 h incubation in 50 mM Tris-HCl (pH 7.4). To detect fibrinogen-degrading enzymes fibrinogen-containing gel was used. Activity of protease/activator was visualized in the gel as clear bands against the dark background. These new techniques offer several advantages including determination of the quantity and activity of t-PA and urokinase, however cannot be recommended for precise quantification of activators; the total procedure is quite quick and simple; method is convenient tool for detection of novel protein-protein interactions in haemostasis system; the sensitivity of the method is ≤0.01 IU per track.