TITLE:
Phenolic Compounds Hybrid Detectors
AUTHORS:
Jadwiga Sołoducho, Joanna Cabaj
KEYWORDS:
Biosensors; Phenolic Compound; Laccase; Tyrosinase; Horseradish Peroxidase; Thin Film; Immobilization; Conducting Polymers
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.4 No.3A,
June
26,
2013
ABSTRACT: Phenolic compounds are among the
major classes of pollutants produced by industrial and agricultural activities.
The amperometric biosensors have been mainly applied to the determination of
phenolic compounds because of the advantages such as good selectivity, low
cost, and easy automation. Amperometry is a method to measure the electric
current that flows as a result of reactions generated at the electrode. Amperometric
phenol biosensors are most often based on tyrosinase, laccase or horseradish
peroxidase immobilized on the electrode surface. The immobilization of enzymes
into ordered thin materials has attracted considerable attention over the past
few years. The present researches have demonstrated that biomolecules
immobilized in different matrixes retain their functional characteristics to a
large extent. These new materials are of great interest for applications as
biosensors and biocatalysts. Lately, also conducting polymers have attracted
much interest in the development of biological sensors. The electrically
conducting polymers are known as possessing many interesting features, which
allow them to act as excellent materials for immobilization of biomolecules.