TITLE:
Transferability and application of microsatellites (SSRs) from Juniperus communis L. to Juniperus procera Hochst. Ex endl.
AUTHORS:
Demissew Sertse, Oliver Gailing, Nicolas-George Eliades, Reiner Finkeldey
KEYWORDS:
Ethiopia; Juniperus communis; Microsatellites; Transferability
JOURNAL NAME:
Open Journal of Genetics,
Vol.3 No.2,
June
25,
2013
ABSTRACT: Transferability of five nuclear microsatellite markers (Jc-16, Jc-31, Jc-32, Jc-35 and Jc-37) that were originally developed for J.
communis was tested to J. procera. Jc-31 & Jc-37 showed
successful amplifications and polymorphism in J. procera. Jc-35 which
had been reported as polymorphic in J.
communis was monomorphic in J.
procera while the primer pair for Jc-32
failed to record any amplification. The remaining one primer
pair (Jc-16) showed double loci ampli-fication in both J. procera and the control J. communis suggesting further examination of the primer pair and its
binding sites. Genetic variation of
six Ethiopian J. procera populations: Chilimo, Goba, Menagesha-Suba, Wef-Washa,
Yabelo and Ziquala was assessed based on the two polymorphic loci (Jc-31 & Jc-37) in 20 - 24 individuals of each
population. From these two loci, a total of 41 alleles could be retrieved. Two
populations that are located south east of the Great Rift Valley together
harboured 75% of private alleles signifying their deviant geo-ecological zones
and suggesting special consideration for conservation. Chilimo, which is at the
western margin of Juniper habitat in
Ethiopian central highlands scored the highest fixation (FIS = 0.584) entailing lower immigrant
genes and hence higher inbreeding. The AMOVA revealed that 97% of the variation resided within thepopulation while still among
population variation was significant (p