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W. A. Van der Schalie, J. H. Gardner, J. A. Buntle, C. H. T. De Rosa, R. A. Finch, J. S. Reif, R. H. Reuter, L. C. Backer, J. Burger, L. C. Folmar and W. S. Stokes, “Animals as Sentinels of Human Health Hazards of Environmental Chemicals,” Environmental Health Perpectives, Vol. 107, No. 4, 1999, pp. 309-315. doi:10.1289/ehp.99107309

has been cited by the following article:

• TITLE: Analysis of Sheep Lymphocyte Chromosomal Aberrations after Exposition to Chlortetracycline

  AUTHORS: Irena Šutiaková, Václav Šutiak, Mária Tulenková

  KEYWORDS: Antibiotics; Ovine Lymphocytes; Chromosomal Assay; Genotoxicity; Cytotoxicity; Health; Environment

  JOURNAL NAME: Journal of Environmental Protection, Vol.4 No.6A, June 25, 2013

  ABSTRACT: Tetracyclines are broad spectrum antibiotics and one type of various their compounds—chlortetracycline has been successfully used for our cytogenetic studies. We have selected for these studies the chromosomal assay because it is wellknown cytogenetic biomarker, which has been used to assess DNA damage at the chromosomal level. We analyzed the chromosomal aberrations and mitotic index in peripheral lymphocytes of sheep exposed by chlortetracycline chloride in pure tap water. To the food of the experimental group of animals (à n = 6) were added also 0.35 gof preparation Aureovit12 C80 plv. a. u. v. per kg b. w. and day (i.e. 168 mg of chlortetracycline hydrochloride/kg b. w.) daily in food. The frequencies of aberrant cells (ABC) in the experimental and control groups of sheep were stated to differ significantly (P = 0.001). In experimental group, chromatid breaks were the dominant type of chromosomal aberrations. No statistical differences in mitotic index values were found in the both groups (P > 0.05). Increased frequencies of chromosome aberrations in peripheral lymphocytes of sheep exposed by chlortetracycline in food, suggested a potential hazard which needs the attention from the viewpoint of human and animal health. This knowledge is useful for all, for the human and veterinary medicine, as well as for the aquaculture. Many of these chlortetracycline substances for their environmental persistence and chemical unstability are present in the environment for various periods, and as a result, they are responsible for several effects on human and animal health through food chain.