TITLE:
Differential Pulse Voltammetric Studies on Lamivudine: An Antiretroviral Drug
AUTHORS:
Katia Christina Leandro, Josino Costa Moreira, Pércio Augusto Mardini Farias
KEYWORDS:
Lamivudine Determination; HIV; Antiretroviral Drugs; Voltammetric Analysis
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.6A,
June
21,
2013
ABSTRACT: Lamivudine (also known as 3TC) is a dideoxynucleoside
analogue, which undergoes intracellular phosphorylation in the putative active
metabolite, lamivudine triphosphate. Lamivudine triphosphate prevents HIV
replication by competitively inhibiting viral reverse transcriptase.
Lamivudine has been extensively used in the treatment of HIV patients owing to its antiretroviral activity. For the determination
of lamivudine in pharmaceuticals, an analytical methodology using voltammetry was
developed. Lamivudine was reduced at a hanging mercury drop electrode (HMDE) at
–1.16 V vs Ag/AgCl at pH 2.0. The
influence of electroanalytical parameters such as scan rate (20 mV.s–1),
amplitude (50 mV), nature of the support electrolyte (Clark-Lubs), and pH (2.0)
on the voltammetric signal was optimized. Under these optimized
conditions, the method had been validated using pharmaceutical formulations.
The lamivudine peak current varied linearly with its concentration from 1.15 to
10.40 mg.L–1, detection and determination limits of 0.46 and 1.0
mg.L–1, respectively, and recovery of 95.15% with a relative
standard deviation of 1.10%.