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Tominaga, H., Ishiyama, M., Ohseto, F., Sasamoto, K., Hamamoto, T., Suzuki, K. and Watanabe, M. (1999) A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Analytical Communications, 36, 47-50. doi:10.1039/a809656b

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• TITLE: In vitro responses of human pulp cells and 3T3 mouse fibroblasts to six contemporary dental restoratives

  AUTHORS: Jun Sun, Yiming Weng, Fengyu Song, Dong Xie

  KEYWORDS: In Vitro Cytotoxicity; Human Pulp Cells; 3T3 Mouse Fibroblast Cells; Dental Cement; Glass-Ionomer Cement; Resin Composite

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.4 No.1, January 29, 2011

  ABSTRACT: In vitro responses of human primary pulp cells (HPCs) and 3T3 mouse fibroblasts to six contempo-rary commercial dental restoratives were evaluated using the WST-1 assay. The results show that Fuji II is not cytotoxic to both cells. Fuji II LC is not cyto-toxic to HPCs but cytotoxic to 3T3 cells, indicating that 3T3 cells are more vulnerable to 2-hydroxyethyl methacrylate (HEMA) than HPCs. Vitremer is very cytotoxic probably due to having diphenyliodonium chloride and HEMA in it. Z100 is very cytotoxic probably due to having triethylene glycol dimethacry-late (TEGDMA) in it. P60 is cytotoxic but less cyto-toxic than Z100 probably due to no TEGDMA in it. Durelon is the most cytotoxic among the six materials studied probably due to the high cytotoxicity of zinc ions. Additionally, the cytotoxcity of the tested mate-rials was found to be dose-dependent.