TITLE:
Toxicity Mechanism of Emodin on Interstitial Cells of Cajal
AUTHORS:
Cheng Peng, Yanhong Wang, Yunxia Li
KEYWORDS:
Emodin; ICC; Toxicity; Mechanism
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.4 No.3,
June
20,
2013
ABSTRACT: Aim: To explore the emodin’s toxicity and action
mechanism on the function of interstitial cells of Cajal (ICC) cultured in vitro. Methods: ICC of KM mouse was cultured in vitro. The minimum toxicity concentration and critical time
points of emodin were investigated with Uniform Design methodology and MTT
assay. The cell enzymology assay and enzyme immunoassay (EIA) were applied to
observe the effect of emodin on membrane stability, cellular internal environment, energy metabolism and second messenger
of ICC. Results: The minimum
toxicity concentration was 0.001%, and the critical time points were 30 s, 1 min,
30 min, and 60 min.
After administration of emodin, the damage on cells aggravated with time
prolonging. The activity of malonaldehyde (MDA), lactate dehydrogenase (LDH),
and phosphatase in the cell was raised
significantly (P + and
Ca2+ were increased but K+ concentration was decreased. The Na+-K+-ATPase
activity was promoted but Ca2+-ATPase descended. Second messenger as
IP3 and cAMP also became more active. All these changes had statistical
significance (P Conclusion: Emodin had toxicity function on ICC which can
lead to membrane damage, energy metabolism disorder. This mechanism could be related to electrolytes
concentration disorder, inhibited activity of Na+-K+-ATPase
and Ca2+-ATPase, and raised activity of IP3 and cAMP.