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Haddar, A., Hmidet, N., Ghorbel-Bellaaj, O., Fakhfakh- Zouari, N., Sella-mi-Kamoun, A. and Nasri, M. (2011) Alkaline proteases produced by Bacillus licheniformis RP1 grown on shrimp wastes: Application in chitin extraction, chicken feather degradation and as a dehairing agent. Biotechnology and Bioprocess Engineering, 16, 669-678. doi:10.1007/s12257-010-0410-7

has been cited by the following article:

• TITLE: Thermostable alkaline protease production from Bacillus pumilus D-6 by using agro-residues as substrates

  AUTHORS: Bijender Kumar Bajaj, Gaytri Jamwal

  KEYWORDS: Alkaline Protease; Agro-Residues; Bacillus pumilus; Thermostable

  JOURNAL NAME: Advances in Enzyme Research, Vol.1 No.2, June 20, 2013

  ABSTRACT: Proteases due to their wide range of applications in biotechnological processes have been the focus of intense research for many decades. However, from industrial application view point most of the available proteases lack desired properties; therefore, search for better and efficient thermostable alkaline proteases are always on. Bacillus pumilus D-6, isolated from dairy plant soil sample, in the current study produced protease which showed activity and stability at high alkaline pH (8-12) and high temperatures (70˚C-100˚C). Enzyme activity remained unfazed even in presence of inhibitors like Pb2+ and Hg2+ which are considered universal inhibitors of enzyme activity. Besides, the organism successfully utilized crude agriculture based substrates as carbon and nitrogen source and produced substantial enzyme titre.