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N. Shibata and Y. Okawa, “Enzymatic Synthesis of New Oligosaccharides using Mannosyltransferases from Candida Species and their NMR Assignment,” Biological & Pharmaceutical Bulletin, Vol. 33, No. 5, 2010, pp. 895-899. doi:10.1248/bpb.33.895

has been cited by the following article:

• TITLE: Structural Investigation of Cell Wall Mannan Antigen Obtained from Pathogenic Yeast Candida zeylanoides

  AUTHORS: Hidemitsu Kobayashi, Susumu Kawakami, Yukiko Ogawa, Nobuyuki Shibata, Shigeo Suzuki

  KEYWORDS: Candida zeylanoides; Candida guilliermondii; Cell Wall Mannan; Antigenic Factor; Nuclear Magnetic Resonance

  JOURNAL NAME: Open Journal of Medical Microbiology, Vol.3 No.2, June 19, 2013

  ABSTRACT: Antigenicity of pathogenic yeast Candida zeylanoides NBRC 10326 was found to have similar to that of Candida guilliermondii, but it was slightly different, i.e. NBRC 10326 strain cells react strongly with the factor antibodies 1, 4, and 9, and react relatively weakly with factor antibody 5. We have investigated the structural characteristic of cell wall mannan of this strain using a non-degradable method, nuclear magnetic resonance analysis. The results obtained from NMR analysis, the two-dimensional homonuclear Hartmann-Hahn, indicated that C. zeylanoides mannan contains α-1, 3-linked and β-1,2-linked oligomannosyl side chains corresponding to C. guilliermondii specific antigenic factor 9. In addition, it was found that the mannan contains β-1,2-linked oligomannosyl side chains attached to phosphate groups corresponding to anitigenic factor 5, which were not found in C. guilliermondii mannan.