TITLE:
IGF2 ApaI A/G Polymorphism Evaluated in ESRD Individuals as a Biomarker to Identify Patients with New Onset Diabetes Mellitus after Renal Transplant in Asian Indians
AUTHORS:
Kiran Kumar Vattam, Imran Ali Khan, Sireesha Movva, Kamal Kiran Mukkavali, Subhadra Poornima, Pragna Rao, Qurratulain Hasan, Pavani Upendram
KEYWORDS:
PTDM; IGF2 Gene Polymorphism; Diabetes Mellitus; ESRD; New Onset Diabetes Mellitus
JOURNAL NAME:
Open Journal of Nephrology,
Vol.3 No.2,
June
19,
2013
ABSTRACT: Insulin like growth factors2 (IGF2) regulates pancreatic β-cell renewal and apoptosis, which in
turn plays a role in altering insulin activity and glucose homeostasis.
Polymorphisms in IGF2 gene have been associated with altered levels of IGF2.
Hence, ApaI polymorphism in exon 9 of
IGF2 (rs#680) gene was assessed in patients
with end stage renal disease
(ESRD) to identify individuals at risk of developing new onset diabetes
mellitus (NODM) in
Asian Indians. Isolated DNA was used for PCR&RFLP based genotyping of IGF2 ApaI polymorphism which was
carried out in 364 individuals these included 140 patients
who had undergone renal transplant, 42
of which developed new onset diabetes mellitus after renal transplant
and 224 healthy control
volunteers. In the present study NODM or post transplant diabetes mellitus
(PTDM) showed a significant association with G allele and AG genotype
when compared with the Non-NODM ESRD patients after transplant (OR 2.081, 95% CI = 1.191 - 3.634, p = 0.01 and OR 3.188, 95% CI = 1.498 - 6.785, p = 0.002) ESRD patients with healthy
controls also showed an association with G allele and AG genotype (OR 1.512, 95% CI = 1.060 - 2.155, p = 0.02 and OR 2.235, 95% CI = 1.453 - 3.438, p = 0.0002). IGF2
could be used as a biomarker to identify individuals at high risk of developing NODM, it would be a
valuable asset in selecting appropriate immunosuppressive regimens for
individuals undergoing transplant. Present study shows the importance of IGF2 ApaI polymorphism in assessing the risk of NODM in ESRD individuals in
Asian Indians with ESRD.