TITLE:
Arabidopsis Transcription Factor WRKY33 Is Involved in Drought by Directly Regulating the Expression of CesA8
AUTHORS:
Xinjing Wang, Bojing Du, Meng Liu, Na Sun, Xiaoting Qi
KEYWORDS:
WRKY33; Drought; CesA8; Target Gene; Inverse Yeast One-Hybrid Assay
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.6A,
June
19,
2013
ABSTRACT: Arabidopsis (Arabidopsis thaliana)
WRKY33 is a key transcription factor in pathogen-induced defense signaling, but
its function in abiotic stresses remains largely unclear. In this study, we
report on the use of a reverse-genetic approach, as well as a yeast (Saccharomyces
cerevisiae) expression system, to determine the role of WRKY33 in drought. A T-DNA
insertion deletion mutant of WRKY33 is more sensitive to dehydration. Through genome-wide screening the
target genes of WRKY33 in yeast, we identified 23 candidate genes including a drought tolerance gene CesA8. Further results revealed
that WRKY33 repressed CesA8 expression
through binding
to the W-box elements of CesA8 distal promoter region and probably interacting with the
transcriptional activator of CesA8,
MYB46. These findings revealed the primary molecular mechanism underlying the
function of
WRKY33 in response to
drought