TITLE:
An in Vitro Approach for the Conservation of Meizotropis pellita: An Endangered and Endemic Plant
AUTHORS:
Lalit Singh, Tapan Kumar Nailwal, Lalit Tewari
KEYWORDS:
Patwa; Patwadanger; Meizotropis pellita
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.6,
June
11,
2013
ABSTRACT: An efficient
protocol for high frequency in vitro regeneration of Meizotropis pellita an endangered and endemic plant was
developed. In vitro germination of
the seeds of Patwa was achieved successfully after incubation for 10 -15 days and
plantlets up to a height of 5 -7 cm with profuse rooting
were observed after incubation for 7 weeks in hormone free MS medium. This in vitro germination totally reduced
contamination that used to occur in the explant taken from the field. Callus
induction and proliferation were observed in leaf explants after 15 -20 days of
incubation in MS medium containing 2 -4, D (9.06 μM) alone or
in combination with 2 -4, D (9.06 μM) + 2-iP (7.38 μM). Shoot
regeneration was achieved from 2 months old callus explant in MS medium
supplemented with BA (17.6 μM) + GA3 (1.0 μM). Shoot
multiplication was also achieved from cotyledonary node of M. pellita in MS medium supplemented with Kinetin + GA3 (4.6 μM + 1.0 μM) or BA
(13.2, 17.6 μM) + GA3 (1.0 μM) after 30 -45 days of incubation. IBA
(4.9 μM) was more effective in root regeneration from micro shoots. The
plantlets after acclimatization over a period of 1 month were further hardened
in a polyhouse for two months. On the basis of available literature this is the
first and significant study regarding the comparative effect of different PGRs
on in-vitro propagation of Meizotropis pellita by using different explants and their subsequent effect on rooting. This
significant study could be useful for large scale production of successfully
hardened plants and conservation of this shrub.