TITLE:
Proteomics Uncovers a Role for Enhanced Ultraviolet-B Radiation on Wheat Leaves
AUTHORS:
Jiangyan Duan, Xue Tian, Zhenhu Jia
KEYWORDS:
Wheat; Ultraviolet-B Radiation; 2-DE
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.6,
June
11,
2013
ABSTRACT: Wheat (Triticum aestivum), as a kind of important
economic crop cultured in the Northern China, is affected by present-day
enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact
by UV-B radiation on it, the proteins of wheat (Jin mai NO.8) leaves, which were
divided into the normal light group (CK) and UV-B radiation group (B), were
extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins
were also analyzed by run two-dimensional gel electrophoresis (2-DE), which
allowed the identification of some significantly different gel spots. The
proteins spots were further verified by Matrix-Assisted Laser
Desorption/lonization-time of Flight Mass Spectrometry. The results showed: 1) the
enhanced UV-B affects the growth of the wheat, as the visual changes appear on
the sixth day; 2) the proteins expressions between the B group and the CK group
were remarkably different on the sixth day; 3) the proteins of wheat
leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one
protein points were identificated between the B group and the CK group.
Among these twenty-one proteins, six proteins of them were up-regulated and
twelve proteins of them were down-regulated, three new proteins were expressed
only in the B group. Three proteins among six proteins, which were
up-regulated, were further verified as RuBisCo large subunit binding protein;
SOD; Calmodulin. The result indicates wheat could improve genes encoding
proteins in their leaves and protect themselves, when enhanced UV-B affects the
growth of the wheat.