TITLE:
Proteomics: A Successful Approach to Understand the Molecular Mechanism of Plant-Pathogen Interaction
AUTHORS:
Tushar Dilipchand Lodha, Padmalochan Hembram, Nitile Tep, Jolly Basak
KEYWORDS:
Proteome; Plant-Pathogen Interaction; Systemic Acquired Resistance; Mass Spectrometry; Pathogenicity Related Proteins
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.6,
June
11,
2013
ABSTRACT: In recent
years, proteomics has played a key role in identifying changes in protein
levels in plant hosts upon infection by pathogenic organisms and in characterizing cellular and
extracellular virulence and pathogenicity factors produced by pathogens.
Proteomics offers a constantly evolving set of novel techniques to study all
aspects of protein structure and function. Proteomics aims to find out the
identity and amount of each and every protein present in a cell and actual
function mediating specific cellular processes. Structural proteomics
elucidates the development and application of experimental approaches to define
the primary, secondary and tertiary structures of proteins, while functional
proteomics refers to the development and application of global (proteome wide
or system-wide) experimental approaches to assess protein function. A detail
understanding of plant defense response using successful combination of
proteomic techniques and other high throughput techniques of cell biology,
biochemistry as well as genomics is needed for practical application to secure
and stabilize yield of many crop plants. This review starts with a brief
introduction to gel- and non gel-based proteomic techniques followed by the
basics of plant-pathogen interaction, the use of proteomics in recent pasts to
decipher the mysteries of plant-pathogen interaction, and ends with the future
prospects of this technology.