TITLE:
A Study into Digital Dermatitis Transmission and Bacterial Associated Pathological Changes Involved in the Disease
AUTHORS:
N. Capion, M. Boye, C. Ekstrøm, K. Dupont, T. K. Jensen
KEYWORDS:
Digital Dermatitis; Treponema spp.; Histopathology; Infection Dynamics
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.3 No.2,
June
11,
2013
ABSTRACT: Digital
dermatitis is a highly prevalent painful lesion affecting the feet in dairy
cattle. Even though the pathogenesis has been subject of investigation since
1974, there is still a lack of knowledge about the spread of the disease among
cows within a herd as well as between herds. The purpose of this study was to
monitor transmission of digital dermatitis under experimental conditions
between naive heifers and affected animals, to monitor the changes in clinical
appearance, microbial colonisation of the skin as lesions progressed and to
apply a q-PCR for the detection of Treponema spp. in faecal samples. Eight heifers with clinical normal digital skin were
housed with 5 heifers with severe digital dermatitis lesion for 8 weeks on a solid concrete floor with an
accumulating layer of slurry. Digital skin was examined daily and lesions were
clinically scored. Skin biopsies were taken from the healthy heifers at
introduction and weekly from all lesions for histopathological evaluation and
fluorescence in situ hybridization. None of the healthy heifers developed
digital dermatitis and in 4 out of 5 infected heifers the lesions healed during
the study. All samples from healthy skin were negative for Treponema spp. and one sample were positive for Dichelobacter nodosus. Colonization of healthy skin could not be identified in
this study. There was no significant relation between clinical scoring of the
lesions and histopathological score and the presence of Treponema spp. There were however a significant relation between the prevalence of Treponema spp. in the skin and severity of changes in epidermis and
dermis. By qPCR all the healthy heifers were found to excrete Treponema spp. in their faeces.