TITLE:
Improved Micropropagation in Hybanthus enneaspermus L. Muell
AUTHORS:
Gandhi Premkumar, Neelakantan Arumugam, Sankaralingam Muthuramkumar, Govidaraju Varatharaju, Kaniappan Rajarathinam
KEYWORDS:
Hybanthus enneaspermus; Multiple Shoots; Regeneration; Morphology
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.6,
June
10,
2013
ABSTRACT: Multiple shoots were induced on
the cut ends of the leaf of Hybanthus
enneaspermus on MS medium fortified with different concentration of
cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the
multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in
combination with KI + BAP. The regenerated shoots show
two different morphological leaves. Shoots are 100% rooted on half-strength MS medium supplemented
with 2.46 μM·l-1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization
and they appeared morphologically normal.