TITLE:
Plasma and Red Blood Cells Concentration Profiles of Ktamine after Single Intravenous Administration in an Anaesthetic Protocol in Horses
AUTHORS:
Francesca Sori, Noemi Romagnoli, Domenico Ferrara, Anna Zaghini, Paola Roncada
KEYWORDS:
Ketamine; Horse; Plasma; Red Blood Cells; Kinetics
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.3 No.2,
June
6,
2013
ABSTRACT: The aim of this study was to describe the
concentration profile of ketamine in plasma and red blood cells following an
intravenous (IV) bolus in the horse. Ten healthy standardbred horses (two males and height females) 7.7 ± 4.6
(mean value ± SD) years old and weighting 380 ± 21 kg (mean value ± SD) were recruited. The horses were premedicated with acepromazine
(0.04 mg·kg-1·IV). Fifteen minutes later they received romifidine
(0.08 mg·kg-1·IV), and 5 minutes after they were
administered midazolam (0.06 mg·kg-1·IV). Immediately, anaesthesia
was induced by ketamine (2.2 mg·kg-1·IV). Venous blood samples were collected at scheduled time points.
Plasma and red blood cells (RBCs) concentration of ketamine was assayed using a
high performance liquid chromatographic method (HPLC/UV-DAD). The high mean
recovery rates, the high sensitivity, the good linearity, suggest a clinical applicability of
the analytical method. A bicompartmental model resulted as the most appropriate
to describe the ketamine concentration—time profile for both plasma and
RBCs. The fitted regression line between ketamine
plasma concentrations and RBC concentrations supports the good correlation between ketamine concentrations in
plasma and in RBCs. The kinetic
parameters of ketamine calculated for RBC are equal or very similar to the
plasma ones. The study confirms the kinetic behaviour of ketamine used in the horse as anaesthetic inducers
in routine surgery. Finally, the bicompartmental model well describes the
ketamine profile also in RBCs, that it is very close to the
plasma profile, underlining the great importance of RBCs as blood
subcompartment.