TITLE:
The Expression of TIMP1, TIMP2, VCAN, SPARC, CLEC3B and E2F1 in Subcutaneous Adipose Tissue of Obese Males and Glucose Intolerance
AUTHORS:
Dmytro Minchenko, Oksana Ratushna, Yulia Bashta, Ruslana Herasymenko, Oleksandr Minchenko
KEYWORDS:
Obesity; Glucose Intolerance; Men; Gene Expression; TIMP1; TIMP2; VCAN; SPARC; CLEC3B; E2F1
JOURNAL NAME:
CellBio,
Vol.2 No.2,
June
4,
2013
ABSTRACT: We investigated the expression of TIMP1, TIMP2, SPARC, VCAN, and CLEC3B genes, encoded
matricellular proteins with pleiotropic functions, and glucose intolerance in
obese male subjects with normal and impaired glucose tolerance. The purpose of this study
was to examine the association between the gene expressions and
glucose intolerance in obesity. The results indicate that obesity leads to significant increase of TIMP1, TIMP2, E2F1 and CLEC3B gene expressions in
subcutaneous adipose tissue, especially TIMP2 gene. However, more significant increase of the expression
of TIMP1 and TIMP2 was found in adipose
tissue of obese patients with glucose intolerance. No significant changes were
found in the expression of VCAN and SPARC genes in adipose tissue of obese subjects with normal glucose tolerance but
increased in the group of obese subjects with glucose intolerance. At the same
time, the E2F1
and CLEC3B gene expressions were
decreased in adipose tissue of obese patients with glucose intolerance. Results
of this study provide evidence that changes in the expression of genes encoded TIMP1, TIMP2, VCAN, SPARC, E2F1 and CLEC3B in subcutaneous adipose tissue of obese individuals associate with
glucose intolerance.