TITLE:
Nanoparticles Production and Inclusion in S. aureus Incubated with Polyurethane: An Electron Microscopy Analysis
AUTHORS:
Lyubov V. Didenko, George A. Avtandilov, Natalia V. Shevlyagina, N. M. Shustrova, Tatiana A. Smirnova, I. Y. Lebedenko, Roberta Curia, Claudio Savoia, Francesco Tatti, Marziale Milani
KEYWORDS:
Polyurethane; S. aureus; Biodestruction; Endocytosis; FIB/SEM (Focused Ion Beam/Scanning Electron Microscope); TEM (Transmission Electron Microscope); STEM (Scanning Transmission Electron Microscope)
JOURNAL NAME:
Open Journal of Medical Imaging,
Vol.3 No.2,
June
4,
2013
ABSTRACT: This
study shows that submicron/nanoparticles found in bacterial cells (S.
aureus) incubated with polyurethane (a material
commonly used for prostheses in odontostomatology) are a consequence of biodestruction. The
presence of polyurethane nanoparticles into bacterial vesicles suggests that
the internalization process occurs through endocytosis. TEM and FIB/SEM are a
suitable set of correlated instruments and techniques for this multi facet
investigation: polyurethane particles influence the
properties of S.
aureus from the morpho-functional standpoint that
may have undesirable effects on the human body. S. aureus and C. albicans are symbiotic microorganisms;
it was observed that C. albicans has
a similar interaction with polyurethane and an
increment of the biodestruction capacity is expected by its mutual work with S. aureus.