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M. Piraud, C. Vianey-Saban, K. Petritis, C. Elfakir, et al., “ESI-MS/MS Analysis of Underivatised Amino Acids: A New Tool for the Diagnosis of Inherited Disorders of Amino Acid Metabolism. Fragmentation sTudy of 79 Molecules of Biological Interest in Positive and Negative Ionisation Mode,” Rapid Communications in Mass Spectrometry, Vol. 17, No. 12, 2003, pp. 1297-1311. doi:10.1002/rcm.1054

has been cited by the following article:

• TITLE: A New Metal Tag for Highly Selective and Sensitive Analyses of Amino Acids and Dipeptides by HPLC/ICP-MS

  AUTHORS: Daigo Iwahata, Kazuki Nakamura, Rie Yamada, Hiroshi Miyano, Naoyuki Yamada

  KEYWORDS: Metal Tag; HPLC/ICP-MS; Amino Acid; Dipeptide; Derivatization; Speciation

  JOURNAL NAME: Journal of Analytical Sciences, Methods and Instrumentation, Vol.3 No.2, May 31, 2013

  ABSTRACT: We have developed a novel metal tag, bis(ethylenediamine)-4'-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester (ECRS) for sensitive analysis of amino acids using high performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS). ECRS is a functional reagent, containing an ester group at one end that can be activated to bind to amino group and a chelated ruthenium at the other. The activated ester was reacted briefly with amino groups under weakly alkaline conditions. The ruthenium was detected sensitively by ICP-MS. ECRS was reacted with 17 proteinogenic amino acids in borate buffer. The derivatives were separated by reversed phase HPLC and identified by quadrupole-based ICP-MS. ECRS was suitable for speciation; low molecular weight compounds containing amino groups. We have thus established a quantitative analytical method for amino acids and dipeptides. The detection limits of branched amino acids (signal-to-noise ratio of 3) were 1.5 nmol·L-1 in the standard solution (100 amol per injection).