TITLE:
Research of Quality Standards for Stachydrine Hydrochloride in Chinese Medicine TJF Granule
AUTHORS:
Yao Lu, Wei Yu, Pingniang Shen, T. J. Khoo, C. Wiart
KEYWORDS:
TJF Granule; Quality Standards; Traditional Chinese Medicine; Evaporative Light Scattering Detector (ELSD); High-Performance Liquid Chromatographic (HPLC) Method; Stachydrine Hydrochloride; Pharmaceutical Analysis; Determination
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.4 No.3,
May
31,
2013
ABSTRACT: A
high-performance liquid chromatographic method was applied to the determination
of stachydrine hydrochloride concentration in TJF granule (Chinese name:
Tiao-Jing-Fang), using a mobile phase of methanol-acetonitrile (50:50, v/v) by the Agilent
Kromasi NH2 column (250 mm × 4.6 mm, 5 μm, S/N: 22N25110). Detection wavelength was 201
nm. The result revealed good linearity of stachydrine hydrochloride and was
obtained within the range of 0.20 - 1.98 μg/mL (R = 0.9995). The average recovery was 97.01%; the relative standard deviation (RSD) was 0.19%. To the best of our knowledge,
this is the first report dedicated to the determination of stachydrine
hydrochloride by the evaporative light scattering detector-high-performance
liquid chromatographic (ELSD-HPLC) method.