TITLE:
Preliminary Report of PGR’s Influence to Multiple Shoots Induction and Plant Regeneration on Plumbago auriculata
AUTHORS:
Yi Chen, Suping Gao
KEYWORDS:
Plumbago auriculata; Multiple Shoots; Induction; Plant Regeneration; PGR
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.5A,
May
30,
2013
ABSTRACT: With the
explants of young nodals and leaves of Plumbago
auriculata, we studied multiple shoots induction and plant regeneration in
the medium with different PGR proportions. The results showed the nodals of Plumbago auriculata could be used as the
explant to induce multiple shoots. After culturing young nodals for 30 d,
optimum single shoot formation was achieved on MS medium supplemented with 0.3 mg/L
6-BA and the induction rate was 70.79%. After being transferred to the medium
containing 1.0 mg/L 6-BA, 1.0 mg/L NAA and 0.2 mg/L IAA for 1 - 2 generation,
single shoot was induced to form multiple shoots with the multiplication
rate was 506.45%. After multiple shoots grew to 3
cm high, the multiple shoots were cut into single shoots and
transferred onto half-strength MS medium supplemented with 0.5 mg/L NAA and the
rooted rate was 94.33%. Finally the intact plants were obtained.