TITLE:
An Approach for Micropropagation of Blueberry (Vaccinium corymbosum L.) Plants Mediated by Temporary Immersion Bioreactors (TIBs)
AUTHORS:
Ariel D. Arencibia, Carolina Vergara, Karla Quiroz, Basilio Carrasco, Carmen Bravo, Rolando Garcia-Gonzales
KEYWORDS:
Temporary Immersion Bioreactors; Vaccinium corymbosum L.; Photomixotrophic; Blueberry; Micropropagation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.5,
May
29,
2013
ABSTRACT: A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation
in programmed Temporary Immersion Bioreactors (TIBs based on two separate
bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and
Brillita. Plant cultures were developed in a controlled environment with 0.4
MPa CO2 enrichment, sucrose-reduced medium, and light intensity of
60 mM m-2·s-1.
Principal component analysis showed that component 1 (C1) grouped 64.08% of the
total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated
three clusters corresponding with the blueberry genotypes, and within each
cluster plants micropropagated in agar-base medium grouped separately from
those plants multiplied in TIBs. Both plant number and total internodes traits
(related to the productive efficiency) were demonstrated superior in
blueberries propagated in TIBs. Additionally, when transferred to
greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those
cultured by the conventional approach, altogether evidencing the occurrence of
a photomixotrophic stage in the vitroplantlets
cultured in TIBs.