TITLE:
Isolation and expression analysis of CBF4 from Vitis amurensis associated with stress
AUTHORS:
Chang Dong, Meng Zhang, Zhiying Yu, Junpeng Ren, Yang Qin, Bailin Wang, Lizhen Xiao, Zhen Zhang, Jianmin Tao
KEYWORDS:
Vitis amurensis; CBF4; Stress; Transcript Accumulation
JOURNAL NAME:
Agricultural Sciences,
Vol.4 No.5,
May
28,
2013
ABSTRACT: The C-repeat Binding
Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a
complete cDNA) and its promoter from Vitis
amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript
accumulation of V. amurensis increased
under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA)
treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles
under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent
manner. The presence of the cis-elements
MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this
promoter is a component of the CBF transduction pathway, which is involved in
plant response to multistress.