TITLE:
Cyclic nucleotide phosphodiesterase 3B is connected to osteopontin and protein kinase CK2 in pancreatic β-cells
AUTHORS:
Emilia Heimann, Amitabh Sharma, Nalini Raghavachari, Vincent C. Manganiello, Lena Stenson, Eva Degerman
KEYWORDS:
Diabetes Mellitus; Pancreatic Islets; β-Cells; cAMP; Cyclic Nucleotide Phosphodiesterases; PDE; Osteopontin; Protein Kinase CK2
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.6 No.5A,
May
28,
2013
ABSTRACT: Islets from
RIP-PDE3B mice, exhibiting β-cell specific overexpression of the cAMP/cGMP-degrading enzyme phosphodiesterase 3B
(PDE3B) and dysregulated insulin secretion, were subjected to microarray
analysis. We show that osteopontin (OPN) mRNA is increased in a dose-dependent
manner in islets from RIP-PDE3B mice, as compared to wild-type islets. In
addition, in silico analysis shows that PDE3B and OPN are
interacting. Furthermore, OPN interacts with protein kinase CK2 ina distinct submodule of the
protein-protein interaction network. We studied PDE3B and OPN proteins and, in
some cases, also PDE1B and PDE4C,
under conditions of relevance for insulin secretion. In the presence of
forskolin, PDE inhibitors, insulin, or a protein kinase CK2 inhibitor, similar alterations in protein levels
of PDE3B and OPN are shown. In summary, results from using a number of
strategies demonstrate a connection between PDE3B and OPNas well as a role
for protein kinase CK2 inpancreatic β-cells.