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Ziyyat, A., Rubinstein, E., Monier-Gavelle, F., Barraud, V., Kulski, O., Prenant, M. Boucheix, C., Bomsel, M. and Wolf, J.P. (2006) CD9 controls the formation of clusters that contain tetraspaninas and the integrin alpha 6 beta 1, which are involved in human and mouse gamete fusion. Journal of Cell Science, 119, 416-424. doi:10.1242/jcs.02730

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• TITLE: Expression of α6 integrin subunit in bovine oocyte and its potential role during fertilization

  AUTHORS: Roseli Fernandes Gonçalves, Ricardo Pimenta Bertolla, Cássia Maria Barroso Orlandi, Valquiria Hyppolito Barnabe

  KEYWORDS: Integrins; Bovine Oocytes; Fertilization; Sperm-Oocyte Binding

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.4 No.5, May 27, 2013

  ABSTRACT: Fertilization in mammals requires the successful completion of a sequence of steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion to produce a zygote. Although some integrin subunits are known to be associated with the plasma membrane of some mammalian oocytes and spermatozoa, the presence of α6 integrin on bovine oocytes with intact zona pellucida has not been reported. The present study was undertaken to evaluate the expression of α6 integrin subunit in bovine oocyte and to determine if in vitro binding to the zona pellucida and fertilization were affected by treating oocytes with α6 integrin subunit antibody. The α6 integrin subunit was identified on the bovine oocyte by immunocytochemistry. In vitro fertilization was significantly decreased when in vitro matured bovine oocytes were pre-incubated with α6 integrin subunit antibody at concentration 5 and 20 μg/mL, and spermoocyte binding increased. These studies demonstrated the presence of α6 integrin subunit on bovine oocyte, and its importance in fertilization and polyspermy.