TITLE:
Effects of primovaccination and booster vaccination on serum cortisol and humoral immune response in cattle
AUTHORS:
Luis S. L. S. Reis, Paulo E. Pardo, Neusa M. Frazatti-Gallina, Rosana L. Paoli, Eunice Oba, Sergio N. Kronka, Aline S. Camargos
KEYWORDS:
Cattle; Stress; Stressor; Cortisol; Rabies Vaccination; Antibody
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.5,
May
27,
2013
ABSTRACT: The objective of this study was to evaluate the
effect of one or two doses of the anti-rabies vaccination on the serum
concentration of cortisol and the humoral immune response in cattle as well as
the correlation between serum cortisol concentrations and the titers of
rabies-neutralizing antibodies. Nelore cattle were randomly assigned to one of
three groups, which were vaccinated with one dose of rabies vaccine (group
GVSR, N = 15), two doses of rabies vaccine (group GVR, N = 15) or were not
vaccinated (group Gc, N = 15). A commercial liquid inactivated rabies vaccine
was used. The stressors imposed on the cattle were vaccination, corral handling
and the presence of people. Blood samples were collected on days 0, 30 and 60
post-vaccination. Serum cortisol concentrations were determined using a
solid-phase radioimmunoassay, and rabies antibody titers were determined
using a serum neutralization test with BHK21 cells (RFFIT). Both
serum cortisol concentrations and antibody titers increased after the second
(booster) vaccination (P