TITLE:
Simultaneous Quantification of Carbohydrates, Alcohols, and Toxic Components in a Bio-Based Medium Using Dual-Detection HPLC Analysis
AUTHORS:
Majid Soleimani, Lope Tabil
KEYWORDS:
Dual-Detection Analysis; Retention Time; Carbohydrates; Alcohols; Furans
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.5,
May
23,
2013
ABSTRACT: Bio-based media, derived from cellulosic biomass
depolymerisation or bioconversion processes, are composed of several
chemicals and biochemicals. In this study, the main components of a
hemicellulosic-based medium were analyzed using a dual detection HPLC method to
separate and determine concentrations of the major monosaccharides (glucose,
xylose, and arabinose), alcoholic and acidic components (ethanol, xylitol, and
acetic acid) and furanic compounds (furfural and
hydroxymethylfurfural (HMF)). The analyses, which employed a single stationary
phase (Aminex HPX-87H) and two detection methods, were carried out under
isocratic conditions and involved mobile phases consisting of 5 mM sulfuric
acid and acetonitrile in different mix ratios from 0 to 0.061 mole fraction of
acetonitrile. Based on the analysis run time and the chromatogram quality, the
optimum condition was determined for the simultaneous quantification of the
components.