TITLE:
Lipase Inhibitor from Fruits of Solanum stramonifolium Jacq.
AUTHORS:
Watinee Chanmee, Chanya Chaicharoenpong, Amorn Petsom
KEYWORDS:
Solanum stramonifolium Jacq.; Lipase Inhibitor; Carpesterol; Orlistat; Obesity
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.4 No.5,
May
15,
2013
ABSTRACT: Obesity is usually
considered as an overweight or excess body fat, leading to increased health
problems. Obesity is a major risk factor for a number of serious diseases.
Decreasing dietary fat absorption, through inhibition of pancreatic lipase
activity, has been reported to be one of the most effective ways for managing
obesity. The present study was aimed at investigating lipase inhibitors from
edible plants. A lipase inhibitor was isolated from n-hexane and ethyl
acetate extracts of
the ripe fruits of Solanum stramonifolium Jacq. by column chromatography
and identified by spectral analysis. Its structure was elucidated as (22R)-3β-benzoyloxy-22-hydroxy-4α-methyl-5α-stigmast-7-en-6-one or carpesterol (1). Carpesterol
exhibited moderate lipase inhibition activity with IC50 value of
56.0 μg/mL while orlistat, a well- know pancreatic lipase inhibitor, had IC50 value of 3.5 ng/mL.
Moreover, the kinetic properties of carpesterol on pancreatic lipase were evaluated.
Carpesterol is a competitive inhibitor and exhibited antagonistic interaction
when combined with
orlistat on lipase inhibition activity.