TITLE:
Collagen types in relation to expression of estradiol and progesterone receptors in equine endometrial fibrosis
AUTHORS:
Diego Lunelli, Silvana Maris Cirio, Selene C. Leite, Carlos Eduardo Camargo, Luiz Ernandes Kozicki
KEYWORDS:
Endometriosis; Mares; Picrosirius Red; Immunohistochemistry; Estradiol Receptors; Progesterone Receptors
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.4,
April
30,
2013
ABSTRACT: The aim of this study was to determine the influence
of collagen I and III on the expression of estrogen and progesterone receptors in equine endometrial fibrosis. A total of
25 crossbred mares were studies. Two endometrial samples were collected from
each mare,1 inthe estrus and1 inthe diestrus phase. The samples were classified according to histological
changes. Collagen was typed and quantified using the picrosirius red
histochemical technique, and steroid receptors were identified by
immunohistochemistry. The results showed a predominance of collagen type III in
all the endometrial samples. The expression
of estrogen (RE2) and progesterone (RP4) receptors
varied according to the estrous cycle. RE2 and RP4 expression
varied in the estrus and diestrus phases;
there was no influence of collagen I or II on receptor expression.