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Morais, H., Ramos, C., Forgacs, E., Cserhati, T., Oliviera, J. and Illes, T. (2001) Three-dimensional principal component analysis employed for the study of the beta-glucosidase production of Lentinus edodes strains. Chemometrics and Intelligent Laboratory Systems, 57, 57-64. doi:10.1016/S0169-7439(01)00121-6

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• TITLE: Enzymatic activities and kinetic properties of β-glucosidase from selected white rot fungi

  AUTHORS: Priscilla M. Mfombep, Zachary N. Senwo, Omoanghe S. Isikhuemhen

  KEYWORDS: White Rot Fungi; β-Glucosidase; EC 3.2.1.21; Enzymatic Activities; Kinetic Properties; Plant Biomass

  JOURNAL NAME: Advances in Biological Chemistry, Vol.3 No.2, April 30, 2013

  ABSTRACT: Beta-glucosidase is among the suite of enzymes produced by white rot fungi (WRF) to biodegrade plant biomass. This study investigated the enzymatic activities and kinetic properties of β-glucosidase from seventeen WRF comprised of the following species from various geographical locations: Pleurotus ostreatus, Auricularia auricular, Polyporus squamosus, Trametes versicolor, Lentinula edodes, and Grifola frondosa. All the WRF studied showed β-glucosidase activities. Significant variations in protein and carbohydrate contents were also recorded. Beta-glucosidase activities after 30 min of incubation ranged from 6.4 μg (T. versicolor) to 225 μg (G. frondosa). The calculated kinetic constant (Km) ranged from 0.47 μM (A. auricular-1120) to 719 μM (L. edodes-7). The Vmax depending on the kinetic transformation model ranged from 0.21 μg·min-1 (T. versicolor) to 9.70 μg·min-1 (G. frondosa-28). Beta-glucosidase activities also exhibited pH optima between 3.5 and 5.0 while temperature optima were between 60°C and 70°C with some media exhibiting a secondary temperature peak at 90°C attributable to the presence of thermostable isoenzyme. WRF if appropriately screened and purified can be harnessed to potentially improve the bio-conversion of cellulose to glucose and also facilitate efficient plant biomass biodegradation and production of useful plant bio-products.