TITLE:
Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD
AUTHORS:
Shiwei Wang, Min Wang, Qinghui Wang
KEYWORDS:
Rhodococcus ruber; NHase; Mutagenesis; Random Amplified Polymorphic DNA
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.3 No.2,
April
30,
2013
ABSTRACT: A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the
starting strain CGMCC3090 with ultraviolet
irradiation and lithium chloride. The NHase activity
of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml),
as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in
seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).