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Shimizu S, Ogawa, J. and Kataoka, M. (1997) Screening of novel microbial enzymes for the production of biologically and chemically useful compounds. Advances in Biochemical Engineering/Biotechnology, 58, 46-87. doi:10.1007/BFb0103302

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• TITLE: Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD

  AUTHORS: Shiwei Wang, Min Wang, Qinghui Wang

  KEYWORDS: Rhodococcus ruber; NHase; Mutagenesis; Random Amplified Polymorphic DNA

  JOURNAL NAME: American Journal of Molecular Biology, Vol.3 No.2, April 30, 2013

  ABSTRACT: A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).